A simple, sensitive, precise and specific RP-HPLC method was developed and validated for the estimation of Glimepiride in tablet dosage form. The method was performed on a RP-HPLC (Shimadzu LC-2010C HT) model, Symmetry C18- column, 250 mm × 4.6 mm, 5 µm particle size. The mobile phase was mixture of sodium dihydrogen phosphate adjusted to the pH 2.5 with orthophosphoric acid and acetonitrile (40:60) at a flow rate of 1 ml/min at ambient temperature. UV detection was performed at 228 nm. The linear response was observed in the range of 50 to 150µg/ml with a correlation coefficient of 0.999996. System precision and method precision were found to be within limits and also ruggedness. The proposed method has adequate specificity for the estimation of Glimepiride in tablet dosage form. The percentage recovery was found to be between the ranges of 99.33% to 100.59%. The method was validated statistically and was applied successfully for the estimation of Glimepiride in tablet dosage form.
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